This is a short listing of Drosophila protocols available on the Web, from a variety of Drosophila labs. If you would like to contribute a protocol, please write to me; if you don't have a Web server, pages can be hosted here.

General

Fly Handling

• Working with Drosophila, from the Bloomington Stock Center.
• More recipies from Applied Scientific.
• Quarantine of new stocks (Todd Laverty, Rich Carthew)
• Fly Husbandry (Grace Panganiban)
• Media recipies from Bloomington Stock Center.
• Fly Culturing, from Bloomington.

Staining

• Embryo Fixation (Ilan Davis).
• Immunostaining Drosophila Embryos (Rich Carthew).
• Embryo antibody stains (Grace Panganiban)
• Fluorescent in situ hybridization (Henry Krause)
• RNA In situ protocol (Ilan Davis)
• In situ RNA stains with digoxygenin (Grace Panganiban).
• In situ/Antibody double labeling in embryos (Henry Krause).
• Another in situ/Antibody protocol from the Posakony lab (PDF).
• Making mesh baskets to collect embryos for staining (Rich Carthew)
• Pre-absorption of antibodies using fixed embryos (Rich Carthew).

• Antibody staining of imaginal discs (Grace Panganiban).
• Antibody Stain of imaginal disks (Cooley Lab/Thom Kauffman).
• Antibody stain of laval imaginal disks (Cooley lab).
• Pupal staining with BrdU.
• In situ RNA hybridization for Imaginal Discs.
• Antibody/In situ double staining of imaginal discs.
• Xgal staining of discs (Grace Panganiban).
• Immunostaining of polytene chromosomes and immunostaining plus in situ hybridization, by Giancomo Cavalli (PDF format).

• Microscopic Analysis of the Adult Retina
• Adult Head Frozen Tissue Sections
  • B-Galactosidase Activity Staining
  • Antibody Staining
• Antibody Staining of Adult Retina
• General whole-mount stain.
• Antibody stain of ovaries (Lynn Cooley)
• Testis staining (Amy Kiger).

• Chromosome In Situ Hybridization Using Biotin Labeled Probes
• Whole Mounts of Pupal Retinas
• Cuticle preparations
• The Developmental Studies Hybridoma Bank has many specific monoclonal antibodies of interest.
• The Antibody Resource Page
• Two-colour FISH on embyros or imaginal discs, from Giacomo Cavalli.

Genetics

• X Ray Mutagenesis
• Embryo Transformation (Eugen Buehler/ Rich Carthew)
• Germline embryo transformation (Scott Barolo)
• Embryo Transformation (PDF format, by Jerome Dejardin and Giacomo Cavalli).
• Generating Somatic Clones by FRT Recombination
• Somatic Mosaic Analysis in the Eye
• Protocols and background biology of P elements (Bill Engels)
• RNAi in embryos (Rich Carthew).

Molecular Biology

• Drosophila DNA microarrays by Kevin White at Stanford, lists protocols and plans for making DNA arrays from Drosophila genes.
• Formaldehyde cross-linking and chromosomal immunoprecipitation (to identify transcription factor binding sites) by Giacomo Cavalli, Valerio Orlando and Renato Paro (PDF format).
• horizonpress.com has a good set of protocol links.
• The Ambros lab has many good general protocols amonst their C. elegans collection.
• Affinity chromatography
• Far Western blotting
• Rapid Fly DNA prep (for PCR).
• BDGP protocols for cDNA library construction, DNA isolation from P1 clones, in situ hybridisation and P element sequencing by inverse PCR.
• Head, RNA and DNA preps from the Vesicle site at Stanford.
• Chromatin Immunoprecipitation from Giacomo Cavalli

Plasmid Maps

• Vector maps at FlyBase.
• Drosophila vector sequences (Stephen Misener's Vector Sequence Database)
• Maps of P1 vectors used in the Drosophila genome project

Tissue Culture

• Maintenance of imaginal disc cell lines
• Drosophila Cell Lines
• Maintenance of the cl8+ (clone 8) and other imaginal cell lines.

Acknowledgements